Physicochemical features of rhodanese: A review
نویسندگان
چکیده
منابع مشابه
Active site structural features for chemically modified forms of rhodanese.
In the course of the reaction catalyzed by rhodanese, the enzyme cycles between two catalytic intermediates, the sulfur-free and the sulfur-substituted (persulfide-containing) forms. The crystal structure of sulfur-free rhodanese, which was prepared in solution and then crystallized, is highly similar to that of sulfur-substituted enzyme. The inactivation of sulfur-free rhodanese with a small m...
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15 صفحه اولReversible Folding of Rhodanese
For the first time completely reversible unfolding was achieved for guanidinium chloride-denatured rhodanese using a systematically defined protocol. These conditions included fl-mercaptoethanol, lauryl maltoside, and sodium thiosulfate. All components were required to get more than the previous best reactivation with lauryl maltoside of 17% (Tandon, S., and Horowitz, P. (1986) J. Biol. Chem. 2...
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ژورنال
عنوان ژورنال: African Journal of Biotechnology
سال: 2004
ISSN: 1684-5315
DOI: 10.5897/ajb2004.000-2071